| # | Rider | Team | Nationality | Age | Pos. |
| José Luis Arrieta | Ag2r–La Mondiale |  Spain | 38 | |
| Cyril Dessel | Ag2r–La Mondiale |  France | 34 | |
| Hubert Dupont | Ag2r–La Mondiale | France | 28 | |
| Vladimir Efimkin | Ag2r–La Mondiale |  Russia | 27 | |
| Stéphane Goubert | Ag2r–La Mondiale | France | 39 | |
| Lloyd Mondory | Ag2r–La Mondiale | France | 27 | |
| Rinaldo Nocentini | Ag2r–La Mondiale |  Italy | 31 | |
| Christophe Riblon | Ag2r–La Mondiale | France | 28 | |
| Nicholas Roche
→  Irish Road Champion | Ag2r–La Mondiale |  Ireland | 24 | |
| Maxime Bouet | Ag2r–La Mondiale | France | 22 | |
| Sylvain Calzati | Ag2r–La Mondiale | France | 29 | |
| Brice Feillu | Ag2r–La Mondiale | France | 23 | |
| Romain Feillu | Ag2r–La Mondiale | France | 25 | |
| Eduardo Gonzalo | Ag2r–La Mondiale | Spain | 25 | |
| David Lelay | Ag2r–La Mondiale | France | 29 | |
| Geoffroy Lequatre | Ag2r–La Mondiale | France | 27 | |
| Christophe Moreau | Ag2r–La Mondiale | France | 38 | |
| Nicolas Vogondy | Ag2r–La Mondiale | France | 31 | |
| Lance Armstrong | Astana |  United States | 37 | |
| Alberto Contador
→  Spanish Time Trial Champion | Astana | Spain | 26 | |
| Andreas Klöden | Astana |  Germany | 34 | |
| Levi Leipheimer | Astana | United States | 35 | |
| Dmitriy Muravyev | Astana |  Kazakhstan | 30 | |
| Sérgio Paulinho | Astana |  Portugal | 29 | |
| Yaroslav Popovych | Astana |  Ukraine | 29 | |
| Grégory Rast | Astana |  Switzerland | 29 | |
| Haimar Zubeldia | Astana | Spain | 32 | |
| Yukiya Arashiro | Bbox Bouygues Telecom |  Japan | 24 | |
| William Bonnet | Bbox Bouygues Telecom | France | 26 | |
| Pierrick Fédrigo | Bbox Bouygues Telecom | France | 30 | |
| Saïd Haddou | Bbox Bouygues Telecom | France | 26 | |
| Laurent Lefèvre | Bbox Bouygues Telecom | France | 32 | |
| Alexandre Pichot | Bbox Bouygues Telecom | France | 26 | |
| Pierre Rolland | Bbox Bouygues Telecom | France | 22 | |
| Yuri Trofimov | Bbox Bouygues Telecom | Russia | 25 | |
| Thomas Voeckler | Bbox Bouygues Telecom | France | 30 | |
| David Arroyo | Caisse d'Epargne | Spain | 29 | |
| Rui Costa | Caisse d'Epargne | Portugal | 23 | |
| Arnaud Coyot | Caisse d'Epargne | France | 28 | |
| Iván Gutiérrez | Caisse d'Epargne | Spain | 30 | |
| Luis Pasamontes | Caisse d'Epargne | Spain | 29 | |
| Óscar Pereiro | Caisse d'Epargne | Spain | 31 | |
| José Joaquín Rojas Gil | Caisse d'Epargne | Spain | 24 | |
| Luis León Sánchez | Caisse d'Epargne | Spain | 25 | |
| Rigoberto Urán | Caisse d'Epargne |  Colombia | 22 | |
| 1 | Carlos Sastre | Cervélo TestTeam | Spain | 34 | |
| Iñigo Cuesta | Cervélo TestTeam | Spain | 40 | |
| José Angel Gomez Marchante | Cervélo TestTeam | Spain | 29 | |
| Volodymir Gustov | Cervélo TestTeam | Ukraine | 32 | |
| Heinrich Haussler | Cervélo TestTeam | Germany | 25 | |
| Thor Hushovd | Cervélo TestTeam |  Norway | 31 | |
| Andreas Klier | Cervélo TestTeam | Germany | 34 | |
| Brett Lancaster | Cervélo TestTeam |  Australia | 29 | |
| Hayden Roulston | Cervélo TestTeam |  New Zealand | 28 | |
| Stéphane Augé | Cofidis | France | 34 | |
| Samuel Dumoulin | Cofidis | France | 28 | |
| Leonardo Duque | Cofidis | Colombia | 29 | |
| Bingen Fernández | Cofidis | Spain | 36 | |
| Christophe Kern | Cofidis | France | 28 | |
| Sébastien Minard | Cofidis | France | 27 | |
| Amaël Moinard | Cofidis | France | 27 | |
| David Moncoutié | Cofidis | France | 34 | |
| Rémi Pauriol | Cofidis | France | 27 | |
| Igor Antón | Euskaltel–Euskadi | Spain | 26 | |
| Mikel Astarloza | Euskaltel–Euskadi | Spain | 29 | |
| Egoi Martínez | Euskaltel–Euskadi | Spain | 31 | |
| Juan José Oroz | Euskaltel–Euskadi | Spain | 28 | |
| Alan Pérez | Euskaltel–Euskadi | Spain | 26 | |
| Rubén Pérez | Euskaltel–Euskadi | Spain | 27 | |
| Amets Txurruka | Euskaltel–Euskadi | Spain | 26 | |
| Gorka Verdugo | Euskaltel–Euskadi | Spain | 30 | |
| Koldo Fernández | Euskaltel–Euskadi | Spain | 27 | |
| Sandy Casar | Française des Jeux | France | 30 | |
| Jérôme Coppel | Française des Jeux | France | 22 | |
| Anthony Geslin | Française des Jeux | France | 29 | |
| Yauheni Hutarovich
→  Belarussian Road Champion | Française des Jeux |  Belarus | 25 | |
| Sébastien Joly | Française des Jeux | France | 29 | |
| Christophe Le Mével | Française des Jeux | France | 28 | |
| Jérémy Roy | Française des Jeux | France | 22 | |
| Benoît Vaugrenard | Française des Jeux | France | 27 | |
| Jussi Veikkanen | Française des Jeux |  Finland | 28 | |
| Julian Dean | Garmin–Slipstream | New Zealand | 34 | |
| Tyler Farrar | Garmin–Slipstream | United States | 25 | |
| Ryder Hesjedal | Garmin–Slipstream |  Canada | 28 | |
| Daniel Martin | Garmin–Slipstream | Ireland | 22 | |
| David Millar | Garmin–Slipstream |  United Kingdom | 32 | |
| Danny Pate | Garmin–Slipstream | United States | 30 | |
| Christian Vande Velde | Garmin–Slipstream | United States | 33 | |
| Bradley Wiggins | Garmin–Slipstream | United Kingdom | 29 | |
| David Zabriskie
→  United States Time Trial Champion | Garmin–Slipstream | United States | 30 | |
| Alessandro Ballan
→  World Road Race Champion | Lampre–NGC | Italy | 29 | |
| Marco Bandiera | Lampre–NGC | Italy | 25 | |
| Marzio Bruseghin | Lampre–NGC | Italy | 35 | |
| Angelo Furlan | Lampre–NGC | Italy | 32 | |
| David Loosli | Lampre–NGC | Switzerland | 29 | |
| Daniele Righi | Lampre–NGC | Italy | 33 | |
| Mauro Santambrogio | Lampre–NGC | Italy | 24 | |
| Marcin Sapa | Lampre–NGC |  Poland | 33 | |
| Simon Špilak | Lampre–NGC |  Slovenia | 23 | |
| Daniele Bennati | Liquigas | Italy | 28 | |
| Roman Kreuziger | Liquigas |  Czech Republic | 23 | |
| Aleksandr Kuschynski | Liquigas | Belarus | 29 | |
| Vincenzo Nibali | Liquigas | Italy | 24 | |
| Franco Pellizotti | Liquigas | Italy | 31 | |
| Fabio Sabatini | Liquigas | Italy | 24 | |
| Brian Bach Vandborg | Liquigas |  Denmark | 27 | |
| Alessandro Vanotti | Liquigas | Italy | 28 | |
| Frederik Willems | Liquigas |  Belgium | 29 | |
| Carlos Barredo | Quick-Step | Spain | 28 | |
| Sylvain Chavanel | Quick-Step | France | 29 | |
| Steven De Jongh | Quick-Step |  Netherlands | 35 | |
| Stijn Devolder | Quick-Step | Belgium | 29 | |
| Jérôme Pineau | Quick-Step | France | 29 | |
| Sébastien Rosseler | Quick-Step | Belgium | 27 | |
| Matteo Tosatto | Quick-Step | Italy | 35 | |
| Jurgen Van de Walle | Quick-Step | Belgium | 32 | |
| | Quick-Step | | | |
| Denis Menchov | Rabobank | Russia | 31 | |
| Stef Clement | Rabobank | Netherlands | 26 | |
| Juan Antonio Flecha | Rabobank | Spain | 31 | |
| Óscar Freire | Rabobank | Spain | 33 | |
| Juan Manuel Gárate | Rabobank | Spain | 33 | |
| Robert Gesink | Rabobank | Netherlands | 23 | |
| Grischa Niermann | Rabobank | Germany | 33 | |
| Joost Posthuma | Rabobank | Netherlands | 28 | |
| Laurens ten Dam | Rabobank | Netherlands | 28 | |
| Cadel Evans | Silence–Lotto | Australia | 32 | |
| Mickaël Delage | Silence–Lotto | France | 23 | |
| Sebastian Lang | Silence–Lotto | Germany | 29 | |
| Matthew Lloyd | Silence–Lotto | Australia | 26 | |
| Staf Scheirlinckx | Silence–Lotto | Belgium | 30 | |
| Greg Van Avermaet | Silence–Lotto | Belgium | 24 | |
| Jurgen Van Den Broeck | Silence–Lotto | Belgium | 26 | |
| Johan Van Summeren | Silence–Lotto | Belgium | 28 | |
| Charles Wegelius | Silence–Lotto | United Kingdom | 31 | |
| Fumiyuki Beppu | Skil–Shimano | Japan | 26 | |
| Simon Geschke | Skil–Shimano | Germany | 23 | |
| Jonathan Hivert | Skil–Shimano | France | 24 | |
| Thierry Hupond | Skil–Shimano | France | 24 | |
| Koen de Kort | Skil–Shimano | Netherlands | 26 | |
| Cyril Lemoine | Skil–Shimano | France | 26 | |
| Piet Rooijakkers | Skil–Shimano | Netherlands | 28 | |
| Albert Timmer | Skil–Shimano | Netherlands | 24 | |
| Kenny van Hummel | Skil–Shimano | Netherlands | 26 | |
| Mark Cavendish | Team Columbia–HTC | United Kingdom | 24 | |
| Bernhard Eisel | Team Columbia–HTC |  Austria | 28 | |
| Bert Grabsch
→ World Time Trial Champion
→  German Time Trial Champion | Team Columbia–HTC | Germany | 34 | |
| George Hincapie | Team Columbia–HTC | United States | 35 | |
| Tony Martin | Team Columbia–HTC | Germany | 24 | |
| Kim Kirchen
→  Luxembourgian Time Trial Champion | Team Columbia–HTC |  Luxembourg | 30 | |
| Maxime Monfort | Team Columbia–HTC | Belgium | 26 | |
| Mark Renshaw | Team Columbia–HTC | Australia | 26 | |
| Michael Rogers
→  Australian Time Trial Champion | Team Columbia–HTC | Australia | 29 | |
| Filippo Pozzato
→  Italian Road Champion | Team Katusha | Italy | 27 | |
| Alexandre Botcharov | Team Katusha | Russia | 34 | |
| Joan Horrach | Team Katusha | Spain | 35 | |
| Serguei Ivanov | Team Katusha | Russia | 34 | |
| Mikhail Ignatiev | Team Katusha | Russia | 22 | |
| Vladimir Karpets | Team Katusha | Russia | 28 | |
| Danilo Napolitano | Team Katusha | Italy | 28 | |
| Nikolai Trusov | Team Katusha | Russia | 23 | |
| Stijn Vandenbergh | Team Katusha | Belgium | 24 | |
| Gerald Ciolek | Team Milram | Germany | 22 | |
| Markus Fothen | Team Milram | Germany | 27 | |
| Johannes Frohlinger | Team Milram | Germany | 24 | |
| Linus Gerdemann | Team Milram | Germany | 26 | |
| Christian Knees | Team Milram | Germany | 28 | |
| Niki Terpstra | Team Milram | Netherlands | 25 | |
| Peter Velits | Team Milram |  Slovakia | 24 | |
| Fabian Wegmann | Team Milram | Germany | 29 | |
| Peter Wrolich | Team Milram | Austria | 35 | |
| Kurt Asle Arvesen
→  Norwegian Road Champion | Team Saxo Bank | Norway | 34 | |
| Fabian Cancellara
→  Swiss Road Champion | Team Saxo Bank | Switzerland | 28 | |
| Gustav Larsson | Team Saxo Bank |  Sweden | 28 | |
| Stuart O’Grady | Team Saxo Bank | Australia | 35 | |
| Andy Schleck
→ Luxembourgian Road Champion | Team Saxo Bank | Luxembourg | 24 | |
| Fränk Schleck | Team Saxo Bank | Luxembourg | 29 | |
| Nicki Sørensen | Team Saxo Bank | Denmark | 34 | |
| Chris Anker Sørensen | Team Saxo Bank | Denmark | 24 | |
| Jens Voigt | Team Saxo Bank | Germany | 37 | |
