| Colletotrichum trifolii | |
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| Species: | C. trifolii |
| Binomial name | |
| Colletotrichum trifolii Bain, (1906) | |
Colletotrichum trifolii is a fungal plant pathogen of alfalfa, causing the disease alfafa anthracnose. It is a biotroph, obtaining nutrients from the living plant cells before forming asexual spores.
It consists of three 'races'. Races 1 and 2 are found in North America, while race 4 is found in Australia. Race 3 is believed to have been a misidentification of another fungal species.
Cultivars which are resistant to C. trifolii infection are used in a attempt to manage the disease.