Tsymbalyuk is a surname. Notable people with the surname include:
- Alexander Tsymbalyuk (born 1976), Ukrainian bass opera singer
- Inna Tsymbalyuk (born 1985), Ukrainian actress, model and beauty pageant titleholder
- Kseniia Tsymbalyuk (born 1997), Russian road and track cyclist
- Yevhen Tsymbalyuk (born 1996), Ukrainian footballer
- Yuriy Tsymbalyuk, Soviet figure skater