A taurochenodeoxycholate 6alpha-hydroxylase (EC 1.14.14.57), (also called taurochenodeoxycholate 6alpha-monooxygenase) is an enzyme with systematic name taurochenodeoxycholate,NADPH:oxygen oxidoreductase (6alpha-hydroxylating). In humans, the multifunction protein CYP3A4 carries out this role among many others, while a separate protein in pigs, CYP4A21, has also been found to catalyze this same reaction.[1][2][3] This enzyme catalyses some hydroxylation reactions of bile acids.[4]
| Taurochenodeoxycholate 6alpha-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.57 | ||||||||
| CAS no. | 105669-85-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Reactions catalysed
editThe bile acid, lithocholic acid, is converted to hyodeoxycholic acid:[5]
The enzyme uses a combination of molecular oxygen and nicotinamide adenine dinucleotide phosphate (NADPH) for the oxidation.
Similarly, taurochenodeoxycholic acid is converted to taurohyocholic acid:[6]
The enzyme is a cytochrome P450 protein containing heme.[7]
See also
editReferences
edit- ↑ Lundell K, Hansson R, Wikvall K (March 2001). "Cloning and expression of a pig liver taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily". The Journal of Biological Chemistry. 276 (13): 9606–12. doi:10.1074/jbc.M006584200. PMID 11113117.
- ↑ Lundell K, Wikvall K (November 2003). "Gene structure of pig sterol 12alpha-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21)". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1634 (3): 86–96. doi:10.1016/j.bbalip.2003.09.002. PMID 14643796.
- ↑ Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annual Review of Biochemistry. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.
- ↑ Kramer W, Sauber K, Baringhaus KH, Kurz M, Stengelin S, Lange G, Corsiero D, Girbig F, König W, Weyland C (March 2001). "Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure". The Journal of Biological Chemistry. 276 (10): 7291–301. doi:10.1074/jbc.M006877200. PMID 11069906.
- ↑ Araya Z, Wikvall K (April 1999). "6alpha-hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1438 (1): 47–54. doi:10.1016/s1388-1981(99)00031-1. PMID 10216279.
- ↑ Araya Z, Hellman U, Hansson R (August 1995). "Characterisation of taurochenodeoxycholic acid 6 alpha-hydroxylase from pig liver microsomes". European Journal of Biochemistry. 231 (3): 855–61. doi:10.1111/j.1432-1033.1995.0855d.x. PMID 7649186.
- ↑ Enzyme 1.14.14.57 at KEGG Pathway Database.
External links
edit- Taurochenodeoxycholate+6alpha-hydroxylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
