Talk:Polymerase stuttering

flawed example

Shouldn't the new sequence be the reverse complement of the old one?

-k —Preceding unsigned comment added by Ketil (talk • contribs) 10:13, 17 April 2008 (UTC)

Yes, it should. It's supposed to look like this: http://nitro.biosci.arizona.edu/courses/EEB600A-2003/lectures/lecture26/figs/slip.jpg

Here's the flawed version if anybody wants to fix it.

An example of the process is denoted below, with P representing a polymerase:

'''''Step 1'''''
 --->P
 ATCGTAGCAAATCGTAA  } Original strand
 ATCGT  } Growing new strand

'''''Step 2'''''
 --------------->P
 ATCGTAGCAAATCGTAA  } Original strand
 ATCGTAGCAAATCGTAA  } Growing new strand

'''''Step 3'''''
                P<- } Polymerase slips back one
 ATCGTAGCAAATCGTAA  } Original strand
 ATCGTAGCAAATCGTAA  } Growing new strand

'''''Step 4'''''
                ->P  } Transcribes another A
  ATCGTAGCAAATCGTAA  } Original strand
 ATCGTAGCAAATCGTAAA  } Growing new strand

Note: Step 3 & 4 is repeated and new nucleotides are added to the 3' end.

--Rajah (talk) 04:40, 23 May 2010 (UTC)

mistake in references

There is a mistake in the references. The original title of the Mauro et al. paper is: "Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs." not "Analysis of polymerase...", and I am not sure whether it concerns polymerases at all. — Preceding unsigned comment added by 212.87.28.249 (talk) 16:51, 10 December 2011 (UTC)