tRNA (cytosine38-C5)-methyltransferase (EC 2.1.1.204), is an enzyme involved in the methylation of transfer RNA which in humans is encoded by the gene TRDMT1 (previously DNMT2). Its systematic name is S-adenosyl-L-methionine:tRNA (cytosine38-C5)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction:
| TRNA (cytosine38-C5)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.204 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- S-adenosyl-L-methionine + cytosine38 in tRNA S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
In particular, the eukaryotic enzyme catalyses methylation of cytosine38 in the anti-codon loop of tRNAAsp(GTC), tRNAVal(AAC) and tRNAGly(GCC).
See also
editReferences
edit- ↑ Goll MG, Kirpekar F, Maggert KA, Yoder JA, Hsieh CL, Zhang X, Golic KG, Jacobsen SE, Bestor TH (January 2006). "Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2". Science. 311 (5759): 395–8. doi:10.1126/science.1120976. PMID 16424344.
- ↑ Jurkowski TP, Meusburger M, Phalke S, Helm M, Nellen W, Reuter G, Jeltsch A (August 2008). "Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism". RNA. 14 (8): 1663–70. doi:10.1261/rna.970408. PMC 2491481. PMID 18567810.
- ↑ Schaefer M, Pollex T, Hanna K, Tuorto F, Meusburger M, Helm M, Lyko F (August 2010). "RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage". Genes & Development. 24 (15): 1590–5. doi:10.1101/gad.586710. PMC 2912555. PMID 20679393.
External links
edit- TRNA+(cytosine38-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
