Spirit blue or Opal blue SS is a dye with formula C37H30ClN3.[1] It is used as an indicator in agar for detection of lipase activity in bacteria.[2]

Spirit blue
Names
IUPAC name
4-[(4-anilinophenyl)-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]-N-phenylaniline hydrochloride
Other names
Opal blue SS, HSDB 466, Solvent Blue 23 monohydrochloride
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.016.766 Edit this at Wikidata
EC Number
  • 218-441-4
UNII
  • InChI=1S/C37H29N3.ClH/c1-4-10-31(11-5-1)38-34-22-16-28(17-23-34)37(29-18-24-35(25-19-29)39-32-12-6-2-7-13-32)30-20-26-36(27-21-30)40-33-14-8-3-9-15-33;/h1-27,38-39H;1H
    Key: JMCKWTQLJNQCTD-UHFFFAOYSA-N
  • C1=CC=C(C=C1)NC2=CC=C(C=C2)C(=C3C=CC(=NC4=CC=CC=C4)C=C3)C5=CC=C(C=C5)NC6=CC=CC=C6.Cl
Properties
C37H30ClN3
Molar mass 552.12 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

References

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  1. "Opal Blue SS". National Center for Biotechnology Information. Archived from the original on 21 December 2016. Retrieved 21 December 2016.
  2. Starr, Mortimer P. (April 4, 1941). "Spirit Blue Agar: A Medium for the Detection of Lipolytic Microorganisms". Science. 93 (2414): 333–334. Bibcode:1941Sci....93..333S. doi:10.1126/science.93.2414.333. PMID 17748334 via CrossRef.