Pentalenolactone D synthase (EC 1.14.13.170, penE (gene), pntE (gene)) is an enzyme with systematic name 1-deoxy-11-oxopentalenate,NADH:oxygen oxidoreductase (pentalenolactone-D forming).[1] It catalyses the following chemical reaction
| Pentalenolactone D synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.170 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The four substrates of this enzyme are 1-deoxy-11-oxopentalenic acid, reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxygen, and a proton. It products are pentalenolactone D, oxidised NADP+, and water.[2] The starting ketone is made as part of the biosynthetic pathway to pentalenolactone in Streptomyces avermitilis.[3]
The enzyme is a Baeyer-Villiger monooxygenase that uses flavin adenine dinucleotide as a cofactor.[1]
See also
edit- Neopentalenolactone D synthase which uses the same starting material but inserts oxygen to form an alternative lactone.
References
edit- 1 2 Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE (March 2011). "Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone". Biochemistry. 50 (10): 1739–54. doi:10.1021/bi1019786. PMC 3051010. PMID 21250661.
- ↑ Enzyme 1.14.13.170 at KEGG Pathway Database.
- ↑ You Z, Omura S, Ikeda H, Cane DE (March 2007). "Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate". Archives of Biochemistry and Biophysics. 459 (2): 233–40. doi:10.1016/j.abb.2006.11.016. PMC 1857356. PMID 17178094.
External links
edit- Pentalenolactone+D+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
