Wikipedia

New York Structural Biology Center

The New York Structural Biology Center (NYSBC) is a facility in New York City that houses both regional and national equipment and specialists for structural biology, including Cryogenic electron microscopy, Nuclear Magnetic Resonance Spectroscopy, X-ray crystallography, and Protein production.[1][2]

New York Structural Biology Center
Established2002; 24 years ago (2002)
Field of research
Structural Biology
DirectorJeffrey Kieft
Staff50[1]
Location89 Convent Avenue, New York City, New York, 10027, United States
40°48′59″N 73°57′07″W / 40.816312°N 73.951813°W / 40.816312; -73.951813
Websitenysbc.org

History

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NYSBC was formed in 1999,[3][4] and opened in 2002,[5] as a centralized facility for structural biology by the consortium of Rockefeller University, Columbia University, Memorial Sloan Kettering Cancer Center, City University of New York, Icahn School of Medicine at Mount Sinai, Albert Einstein College of Medicine, New York University, New York State Department of Health, and Weill Cornell Medical College.[1] The facility is located in Harlem, on the former grounds of the City University of New York's South Campus.[6]

An expansion of the Cryo-EM resources was funded in 2014 by the Simons Foundation.[7] This was followed by funding from the National Institutes of Health in 2018 to establish the National Center for CryoEM Access and Training (NCCAT)[8][9][10] and in 2022 for a National Center for In-situ Tomographic Ultramicroscopy[11]

In 2023, NYSBC and the life science company Syncell partnered for spatial proteomics using mass spectrometry.[12]

In 2025, the NIH and National Science Foundation funded establishing a Center on Macromolecular Dynamics by NMR spectroscopy at NYSBC.[13]

Activities

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NYSBC supports structural biology research using CryoEM and NMR spectroscopy, and X-ray crystallography through the National Synchrotron Light Source II at Brookhaven National Laboratory.[1][14] The CryoEM resources support Single particle analysis,[15] Cryogenic electron tomography,[11] and Microcrystal electron diffraction.[16]

Scientific work at NYSBC includes developing new methods for CryoEM sample characterization,[17] data collection,[18][19] and processing.[20]

NYSBC also trains users in CryoEM,[21][4] including awarding 'merit badges' for learning current best-practice techniques.[22][23]

The facility also hosted the New York Consortium on Membrane Protein Structure (NYCOMPS) facilities for protein expression to support structural genomics of membrane proteins.[24]

References

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  1. 1 2 3 4 "About Us". NYSBC. Retrieved May 19, 2026.
  2. ↑ Kieft, J; Butcher, DS; Soares, AS; Shi, W; Lazo, EO; Stojanoff, V; Shekar, V; Somasundaram, T (2025). "Regional and National Shared Research Resources: Challenges and Opportunities§". Journal of Biomolecular Techniques. 36 (4): 19–24. PMC 12773490. PMID 41502859.
  3. ↑ Lascar, C; Mendelsohn, LD (April 2002). "Multiple database coverage of structural biology". Journal of the Medical Library Association. 90 (2): 253–6. PMC 100774. PMID 11999187.
  4. 1 2 Cossio, Pilar; Eng, Edward T. (January 2025). "The 2024 New York City Integrative Structural Biology Symposium". Trends in Pharmacological Sciences. 46 (1): 1–8. doi:10.1016/j.tips.2024.11.001. PMID 39613530.
  5. ↑ "New York Structural Biology Center". Rockefeller University. Retrieved May 19, 2026.
  6. ↑ Kapner, Hallie (November 1, 2013). "Engineering New York into a STEM Hub". New York Academy of Sciences. Retrieved May 19, 2026.
  7. ↑ "Foundation Supports New York Structural Biology Center". Simons Foundation. September 9, 2014. Retrieved May 19, 2026.
  8. ↑ Britt, Rachel (May 15, 2018). "NIH funds three national cryo-EM service centers and training for new microscopists". NIH. Retrieved May 19, 2026.
  9. ↑ "National Center for CryoEM Access and Training". NCCAT. Retrieved May 19, 2026.
  10. ↑ Eng, Edward T; Zimanyi, Christina; Wei, Hui; Aragon, Mahira; Chua, Eugene YD; Kopylov, Elina; Dubbeldam, Charlie; Castello, Cathleen; Potter, Clinton S; Carragher, Bridget (August 1, 2022). "The National Center for CryoEM Access and Training: Broadening Access to CryoEM Through Centralized Facilities". Microscopy and Microanalysis. 28 (S1): 2972–2973. Bibcode:2022MiMic..28S2972E. doi:10.1017/S1431927622011138.
  11. 1 2 Kopylov, Mykhailo (February 2022). "National Center for In-situ Tomographic Ultramicroscopy at New York Structural Biology Center". Biophysical Journal. 121 (3): 128a. Bibcode:2022BpJ...121..128K. doi:10.1016/j.bpj.2021.11.2066.
  12. ↑ Wu, Jennie (August 31, 2023). "New York Structural Biology Center and Syncell Announce Partnership for Microscopy-Guided Proteomics". Business Wire. Retrieved May 19, 2026.
  13. ↑ "Bruker Announces Orders for NIH- and NSF-Funded NMR Systems from New York Structural Biology Center, University of Delaware and Northwestern University". Business Wire. October 3, 2025. Retrieved May 19, 2026.
  14. ↑ "Completion of NYSBC Microdiffraction Beamline NYX". Defence Technical Information Center. Retrieved May 19, 2026.
  15. ↑ Kim, LY; Rice, WJ; Eng, ET; Kopylov, M; Cheng, A; Raczkowski, AM; Jordan, KD; Bobe, D; Potter, CS; Carragher, B (2018). "Benchmarking cryo-EM Single Particle Analysis Workflow". Frontiers in Molecular Biosciences. 5 50. doi:10.3389/fmolb.2018.00050. PMC 6009202. PMID 29951483.
  16. ↑ Eng, Edward T; Zimanyi, Christina; Decato, Daniel; Miller, Jessalyn G; Chen, Chun-Hsing (September 1, 2025). "Making MicroED Accessible at a CryoEM Center Through Training and Infrastructure". Structural Dynamics. 12 (5_Supplement): A1. doi:10.1063/4.0000793. PMC 12585362.
  17. ↑ Noble, Alex J; Dandey, Venkata P; Wei, Hui; Brasch, Julia; Chase, Jillian; Acharya, Priyamvada; Tan, Yong Zi; Zhang, Zhening; Kim, Laura Y; Scapin, Giovanna; Rapp, Micah; Eng, Edward T; Rice, William J; Cheng, Anchi; Negro, Carl J; Shapiro, Lawrence; Kwong, Peter D; Jeruzalmi, David; des Georges, Amedee; Potter, Clinton S; Carragher, Bridget (May 29, 2018). "Routine single particle CryoEM sample and grid characterization by tomography". eLife. 7 e34257. Bibcode:2018eLife...734257N. doi:10.7554/eLife.34257. PMC 5999397. PMID 29809143.
  18. ↑ Aragon, Mahira; Bobe, Daija; Carpen, Dianne; Castello, Cathleen; Chua, Eugene Y D; de Marco, Alex; Kieft, Jeffrey S; Kopylov, Elina; Kopylov, Mykhailo; Miller, Jessalyn; Owji, Aaron P; Zimanyi, Christina M; Eng, Edward T (July 25, 2025). "A Comprehensive Calibration Standard for CryoEM Workflow Validation". Microscopy and Microanalysis. 31 (Supplement_1) ozaf048.349. doi:10.1093/mam/ozaf048.349.
  19. ↑ Chua, Eugene; Mendez, Joshua; Paraan, Reza; Kuang, Huihui; Maruthi, Kashyap; Eng, Edward; Krit, Shaker; Cheng, Anchi; Potter, Clint; Carragher, Bridget (August 2021). "Testing and implementing a live processing workflow at the New York Structural Biology Center". Microscopy and Microanalysis. 27 (S1): 2296–2297. Bibcode:2021MiMic..27S2296C. doi:10.1017/S1431927621008254.
  20. ↑ Bepler, Tristan; Morin, Andrew; Rapp, Micah; Brasch, Julia; Shapiro, Lawrence; Noble, Alex J.; Berger, Bonnie (November 2019). "Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs". Nature Methods. 16 (11): 1153–1160. doi:10.1038/s41592-019-0575-8. PMC 6858545. PMID 31591578.
  21. ↑ Cossio, Pilar; Eng, Edward T. (January 2025). "The 2024 New York City Integrative Structural Biology Symposium". Trends in Pharmacological Sciences. 46 (1): 1–8. doi:10.1016/j.tips.2024.11.001. PMID 39613530.
  22. ↑ Aragon, Mahira; Carpen, Dianne; Castello, Cathleen; Chua, Eugene Y D; de Marco, Alex; Dubbeldam, Charlie; Eng, Edward T; Kieft, Jeffrey S; Kopylov, Elina; Owji, Aaron P; Zimanyi, Christina M (July 24, 2024). "Developing a Comprehensive CryoEM Cross-Facility Training Curriculum That Adheres to Best Practices Across Diverse Settings". Microscopy and Microanalysis. 30 (Supplement_1) ozae044.443. doi:10.1093/mam/ozae044.443.
  23. ↑ Ilitchev, Alex (July 25, 2023). "New York Structural Biology Center Broadens Access to Cryo-EM". Thermo Fisher. Retrieved May 19, 2026.
  24. ↑ Love, James; Mancia, Filippo; Shapiro, Lawrence; Punta, Marco; Rost, Burkhard; Girvin, Mark; Wang, Da-Neng; Zhou, Ming; Hunt, John F.; Szyperski, Thomas; Gouaux, Eric; MacKinnon, Roderick; McDermott, Ann; Honig, Barry; Inouye, Masayori; Montelione, Gaetano; Hendrickson, Wayne A. (September 2010). "The New York Consortium on Membrane Protein Structure (NYCOMPS): a high-throughput platform for structural genomics of integral membrane proteins". Journal of Structural and Functional Genomics. 11 (3): 191–199. doi:10.1007/s10969-010-9094-7. PMC 3099345. PMID 20690043.
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