RNA-binding protein Nova-1 is a protein that in humans is encoded by the NOVA1 gene .[ 5] [ 6]
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.[ 6] [ 7] CRISPR side effect,[ 8] [ 9] replicated in a subsequent study.[ 10]
1 2 3 GRCh38: Ensembl release 89: ENSG00000139910 – Ensembl , May 20171 2 3 GRCm38: Ensembl release 89: ENSMUSG00000021047 – Ensembl , May 2017↑ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .↑ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .↑ Buckanovich RJ, Yang YY, Darnell RB (Feb 1996). "The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies" . J Neurosci . 16 (3): 1114– 22. doi :10.1523/JNEUROSCI.16-03-01114.1996 . PMC 6578795 PMID 8558240 . 1 2 "Entrez Gene: NOVA1 neuro-oncological ventral antigen 1" .↑ "Neanderthal Gene Variant Alters Neurodevelopment in Human Brain Organoids" . 11 February 2021.↑ Maricic T, Helmbrecht N, Riesenberg S, Macak D, Kanis P, Lackner M, Pugach-Matveeva AD, Pääbo S (2021-10-15). "Comment on "Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment" Science . 374 (6565) eabi6060. doi :10.1126/science.abi6060 . ISSN 0036-8075 . PMID 34648345 . S2CID 238990790 . ↑ Herai RH, Szeto RA, Trujillo CA, Muotri AR (2021-10-15). "Response to Comment on "Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment" Science . 374 (6565) eabi9881. doi :10.1126/science.abi9881 . ISSN 0036-8075 . PMID 34648331 . S2CID 238990560 . ↑ Riesenberg S, Kanis P, Macak D, Wollny D, Düsterhöft D, Kowalewski J, Helmbrecht N, Maricic T, Pääbo S (2023-07-20). "Efficient high-precision homology-directed repair-dependent genome editing by HDRobust" . Nature Methods . 20 (9): 1388– 1399. doi :10.1038/s41592-023-01949-1 ISSN 1548-7091 . PMC 10482697 PMID 37474806 .
Buckanovich RJ, Posner JB, Darnell RB (1993). "Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system". Neuron . 11 (4): 657– 72. doi :10.1016/0896-6273(93)90077-5 . PMID 8398153 . S2CID 22554933 . Buckanovich RJ, Darnell RB (1997). "The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo" . Mol. Cell. Biol . 17 (6): 3194– 201. doi :10.1128/MCB.17.6.3194 . PMC 232172 PMID 9154818 . Lewis HA, Chen H, Edo C, et al. (1999). "Crystal structures of Nova-1 and Nova-2 K-homology RNA-binding domains" . Structure . 7 (2): 191– 203. doi :10.1016/S0969-2126(99)80025-2 PMID 10368286 . Jensen KB, Dredge BK, Stefani G, et al. (2000). "Nova-1 regulates neuron-specific alternative splicing and is essential for neuronal viability" . Neuron . 25 (2): 359– 71. doi :10.1016/S0896-6273(00)80900-9 PMID 10719891 . Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags" . Proc. Natl. Acad. Sci. U.S.A . 97 (7): 3491– 6. Bibcode :2000PNAS...97.3491D . doi :10.1073/pnas.97.7.3491 PMC 16267 PMID 10737800 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899– 903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Dredge BK, Darnell RB (2003). "Nova regulates GABA(A) receptor gamma2 alternative splicing via a distal downstream UCAU-rich intronic splicing enhancer" . Mol. Cell. Biol . 23 (13): 4687– 700. doi :10.1128/MCB.23.13.4687-4700.2003 . PMC 164843 PMID 12808107 . Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol . 22 (6): 707– 16. doi :10.1038/nbt971 . PMID 15146197 . S2CID 27764390 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121– 7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Ule J, Ule A, Spencer J, et al. (2005). "Nova regulates brain-specific splicing to shape the synapse". Nat. Genet . 37 (8): 844– 52. doi :10.1038/ng1610 . PMID 16041372 . S2CID 8446567 . Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration" . Cell . 125 (4): 801– 14. doi :10.1016/j.cell.2006.03.032 PMID 16713569 .
