In enzymology, 12alpha-hydroxysteroid dehydrogenase (EC 1.1.1.176) is an enzyme that catalyzes the chemical reaction
| 12-alpha-hydroxysteroid dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.176 | ||||||||
| CAS no. | 61642-40-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The two substrates of this enzyme are the bile acid, cholic acid, and oxidised nicotinamide adenine dinucleotide phosphate (NADP+). Its products are 12-ketochenodeoxycholic acid, reduced NADPH, and a proton.[1][2][3]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 12alpha-hydroxysteroid:NADP+ 12-oxidoreductase. Other names in common use include 12alpha-hydroxy steroid dehydrogenase, 12alpha-hydroxy steroid dehydrogenase, NAD+-dependent 12alpha-hydroxysteroid dehydrogenase, and NADP+-12alpha-hydroxysteroid dehydrogenase. This enzyme is involved in a metabolic pathway that degrades bile acids into cholesterol.
References
edit- ↑ Enzyme 1.1.1.176 at KEGG Pathway Database.
- ↑ MacDonald IA, Mahony DE, Jellet JF, Meier CE (1977). "NAD-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from Eubacterium lentum ATCC no. 25559". Biochim. Biophys. Acta. 489 (3): 466–76. doi:10.1016/0005-2760(77)90167-9. PMID 201289.
- ↑ Mahony DE, Meier CE, Macdonald IA, Holdeman LV (1977). "Bile salt degradation by nonfermentative clostridia". Appl. Environ. Microbiol. 34 (4): 419–23. Bibcode:1977ApEnM..34..419M. doi:10.1128/aem.34.4.419-423.1977. PMC 242673. PMID 921266.
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