Glycoprotein 6-alpha-L-fucosyltransferase

In enzymology, a glycoprotein 6-alpha-L-fucosyltransferase (EC 2.4.1.68) is an enzyme that catalyzes the chemical reaction

glycoprotein 6-alpha-L-fucosyltransferase
Identifiers
EC no.	2.4.1.68
CAS no.	9033-08-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

GDP-L-fucose + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D- glucosaminyl}asparagine $\rightleftharpoons$ GDP + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L- fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl}asparagine

The 2 substrates of this enzyme are GDP-L-fucose and N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine, and its 2 products are GDP and N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl}asparagine.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-a cetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-man nosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glu cosaminyl}asparagine) 6-alpha-L-fucosyltransferase. Other names in common use include GDP-fucose-glycoprotein fucosyltransferase, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1->6fucosyltransferase, GDP-L-fucose-glycoprotein fucosyltransferase, glycoprotein fucosyltransferase, guanosine diphosphofucose-glycoprotein fucosyltransferase, GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked, N-acetylglucosamine of, 4-N-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-, acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-, mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-, glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase, and FucT. This enzyme participates in 3 metabolic pathways: n-glycan biosynthesis, keratan sulfate biosynthesis, and glycan structures - biosynthesis 1.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes PDB: 2DE0​, PDB: 2HHC​, and PDB: 2HLH​.

References

• Longmore GD, Schachter H (1982). "Product-identification and substrate-specificity studies of the GDP-L-fucose:2-acetamido-2-deoxy-beta-D-glucoside (FUC goes to Asn-linked GlcNAc) 6-alpha-L-fucosyltransferase in a Golgi-rich fraction from porcine liver". Carbohydr. Res. 100: 365–92. doi:10.1016/S0008-6215(00)81049-6. PMID 7083256.
• Voynow JA, Scanlin TF, Glick MC (1988). "A quantitative method for GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha 1--6fucosyltransferase activity with lectin affinity chromatography". Anal. Biochem. 168 (2): 367–73. doi:10.1016/0003-2697(88)90331-4. PMID 3364733.
• Teshima T, Fujii S, Shiba T, Taniguchi N (1996). "Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-->6fucosyltransferase". J. Biol. Chem. 271 (44): 27810–7. doi:10.1074/jbc.271.44.27810. PMID 8910378.