Flavonoid 3′-monooxygenase (EC 1.14.14.82, originally classified as EC 1.14.13.21) is an enzyme that catalyzes the chemical reaction[1]
| Flavonoid 3′-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.82 | ||||||||
| CAS no. | 85340-98-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
a flavonoid + NADPH + H+ + O2 a 3′-hydroxyflavonoid + NADP+ + H2O
For example, naringenin is converted to eriodictyol:
The enzyme uses molecular oxygen and reduced nicotinamide adenine dinucleotide phosphate (NADPH) to insert a hydroxy group into a specific position in one of the benzene rings of the starting material.[2]
It is a cytochrome P450 protein containing heme which acts as an oxidoreductase. The systematic name of this enzyme class is flavonoid,NADPH:oxygen oxidoreductase (3′-hydroxylating). Other names in common use include flavonoid 3′-hydroxylase, flavonoid 3-hydroxylase (erroneous), NADPH:flavonoid-3′-hydroxylase, and flavonoid 3-monooxygenase (erroneous). In palnts including Matthiola incana from which it was first characterised, it is part of the pathway to anthocyanins.[1][3]
References
edit- 1 2 Forkmann G, Heller W, Grisebach H (1980). "Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3′-Hydroxylases". Zeitschrift für Naturforschung C. 35 (9–10): 691–695. doi:10.1515/znc-1980-9-1004.
- ↑ Enzyme 1.14.14.82 at KEGG Pathway Database.
- ↑ Ververidis F, Trantas E, Douglas C, Vollmer G, Kretzschmar G, Panopoulos N (October 2007). "Biotechnology of flavonoids and other phenylpropanoid-derived natural products. Part I: Chemical diversity, impacts on plant biology and human health". Biotechnology Journal. 2 (10): 1214–1234. doi:10.1002/biot.200700084. PMID 17935117.
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