Diaminobutyrate-2-oxoglutarate transaminase (EC 2.6.1.76) is an enzyme that catalyzes the chemical reaction
| Diaminobutyrate-2-oxoglutarate transaminase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.6.1.76 | ||||||||
| CAS no. | 196622-96-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
-2-Amino-4-oxobutanoic_acid.svg)
-2,4-Diaminobutyric_acid.svg)
The two substrates of this enzyme are L-aspartic-4-semialdehyde and glutamic acid. Its products are the (S) enantiomer of 2,4-diaminobutyric acid (DABA) and α-ketoglutaric acid.[1][2] The product DABA in bacteria is subsequently converted to the osmoprotectant, ectoine.[3][4] In other organisms including Acinetobacter baumannii and Haemophilus influenzae it is the precursor to 1,3-diaminopropane.[5][6]
This enzyme belongs to the family of transferases, specifically the transaminases, which transfer nitrogenous groups. The systematic name of this enzyme class is L-2,4-diaminobutanoate:2-oxoglutarate 4-aminotransferase. Other names in common use include L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, 2,4-diaminobutyrate 4-aminotransferase, diaminobutyrate aminotransferase, DABA aminotransferase, DAB aminotransferase, EctB, diaminibutyric acid aminotransferase, and L-2,4-diaminobutyrate:2-oxoglutarate 4-aminotransferase.[7]
References
edit- ↑ Peters P, Galinski EA, Truper HG (1990). "The biosynthesis of ectoine". FEMS Microbiol. Lett. 71 (1–2): 157–162. doi:10.1111/j.1574-6968.1990.tb03815.x.
- ↑ Ono H, Sawada K, Khunajakr N, et al. (1999). "Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata". J. Bacteriol. 181 (1): 91–9. doi:10.1128/JB.181.1.91-99.1999. PMC 103536. PMID 9864317.
- ↑ Kuhlmann AU, Bremer E (2002). "Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp". Appl. Environ. Microbiol. 68 (2): 772–83. Bibcode:2002ApEnM..68..772K. doi:10.1128/AEM.68.2.772-783.2002. PMC 126723. PMID 11823218.
- ↑ Louis P, Galinski EA (1997). "Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli". Microbiology. 143 (4): 1141–9. doi:10.1099/00221287-143-4-1141. PMID 9141677.
- ↑ Ikai H, Yamamoto S (1997). "Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii". J. Bacteriol. 179 (16): 5118–25. doi:10.1128/jb.179.16.5118-5125.1997. PMC 179370. PMID 9260954.
- ↑ Ikai H, Yamamoto S (1998). "Two genes involved in the 1,3-diaminopropane production pathway in Haemophilus influenzae". Biol. Pharm. Bull. 21 (2): 170–3. doi:10.1248/bpb.21.170. PMID 9514614.
- ↑ Enzyme 2.6.1.76 at KEGG Pathway Database.
